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Wichtige Dokumente
CS0750
Caveolea/Rafts Isolation Kit
sufficient for 20 purifications
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About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Verwendung
sufficient for 20 purifications
Versandbedingung
dry ice
Lagertemp.
−20°C
Anwendung
The kit provides an easy method for obtaining fractions enriched with caveolea/rafts proteins from tissue culture cells. This method for the isolation of caveolae and rafts is based on the insolubility of these structures in ice cold non-ionic detergent Triton X-100. The kit includes all reagents for cells lysis, density gradient, and the isolation of caveolea/rafts protein enriched fraction, as well as two markers for caveolea/rafts compartments, anti-caveolin-1 and cholera toxin B subunit-peroxidase (CTB-HRP).
Biochem./physiol. Wirkung
Caveolae and lipid rafts are important cholesterol and sphingolipid-rich structures that represent a subcompartment or microdomain of the plasma membrane and are implicated in cellular transport and signal transduction related events.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Lysis buffer 100 mL
- Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg
- Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL
- Anti-Caveolin-1 antibody produced in rabbit 50 μL
- Triton X-100 2 mL
- OptiPrep Density Gradient Medium 100 mL
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Lagerklassenschlüssel
10 - Combustible liquids
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