CGD1
Cell Growth Determination Kit, MTT based
Synonym(e):
Mitochondrial activity assay
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About This Item
UNSPSC-Code:
12352207
NACRES:
NA.75
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Verpackung
pkg of 1 kit
Qualitätsniveau
Methode(n)
UV/Vis spectroscopy: suitable
protein quantification: suitable
Amax
570 nm
Anwendung(en)
cell analysis
detection
Nachweisverfahren
colorimetric
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.
Anwendung
Cell Growth Determination Kit, MTT based has been used:
- to measure the cell viability of preosteoblastic cells
- in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
- to determine the cell viability of primary cervical epithelial cells
Verpackung
Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.
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Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
Zielorgane
Central nervous system
Lagerklassenschlüssel
3 - Flammable liquids
Flammpunkt (°F)
53.6 °F - closed cup
Flammpunkt (°C)
12 °C - closed cup
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