SSE-5

95122021, fish embryo, Epithelial-like

• UNSPSC-Code: 41106514
• Biologische Quelle: fish embryo
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 55
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified

Eigenschaften

• Produktbezeichnung: SSE-5, 95122021
• Biologische Quelle: fish embryo
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 55
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Fish Sockeye Salmon embryo

Beschreibung der Zelllinie

SSE-5 was initiated in 1964 from pooled embryos of Sockeye Salmon (Oncorynchus nerka). The cell line that is also known as Sockeye Salmon embryo-5, is susceptible to infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicaemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). For replication of salmonid viruses SSE-5 cells should be grown at 18°C.

Anwendung

Isolation of viruses from clinical samples, virus propagation

Nährmedium

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at leas

Sonstige Hinweise

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.