NP cl.25

Reactivity: Anti-Measles Virus Nucleoprotein, 95051114

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myelom: Sp2/0 Ag-14
• Reaktivität: Anti-Measles Virus Nucleoprotein
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG2a
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse Sp2/0 x Mouse BALB/c spleen

Beschreibung der Zelllinie

NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.

Immunogen

Measles virus (strain Hallè)

Anwendung

Diagnosis, study of measles virus infection

Nährmedium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 1-5x100,000 cells/ml; 5% CO₂; 37°C

Sonstige Hinweise

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