BF-11

95050413, Reactivity: Anti-Erythropoietin, human

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Rat x Mouse Hybridoma
• Verpackung: tube of 5 μg 95050413-DNA-5UG; pkg of vial of cells 95050413-1VL
• ImmunoDon: Rat AC1 spleen cells
• Myelom: Sp2/0 Ag-14
• Reaktivität: Anti-Erythropoietin, human
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat ACI spleen x Mouse Sp2/0 Ag-14

Beschreibung der Zelllinie

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

Immunogen

Human erythropoietin

Anwendung

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Nährmedium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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