HSG (HeLa derivative)

95031024

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human cervix
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: EGF, TGF-beta
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human cervix carcinoma

Beschreibung der Zelllinie

The cell line HSG was originally thought to be established from a human submandibular gland from a 54 year old Japanese male. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Nährmedium

MEM +2mM Glutamine +1% Non Essential Amino Acids (NEAA) + 10% New Born Calf Serum (NBCS).

Subkultur-Routine

Split confluent cultures 1:4 to 1:10 ie seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37°C

Sonstige Hinweise

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