QT 6

93120831, quail muscle, Spindle shaped/round

• UNSPSC-Code: 41106514

Eigenschaften

• product name: QT 6, 93120831
• Biologische Quelle: quail muscle
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Spindle shaped/round
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Quail Japanese fibrosarcoma

Beschreibung der Zelllinie

Derived from methylcholanthrene-induced fibrosarcoma of Japanese quail. The cells are susceptible to several viruses including sub group A avian sarcoma, avian encephalomyelitis, Marek′s disease herpes virus and rabies virus.

Anwendung

Virus studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C; subculture at about 75% confluency; cells can be dislodged by tapping flask against the palm of hand; use of 0.5% polyvinylpyrrol

Sonstige Hinweise

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