K1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92030501, human thyroid, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human thyroid
• Beschreibung: Human thyroid carcinoma
• Verpackung: tube of 5 μg 92030501-DNA-5UG; pkg of vial of cells 92030501-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Wild-type p53 tumour suppressor gene
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human thyroid carcinoma

Beschreibung der Zelllinie

Derived from a primary papillary thyroid carcinoma. Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene. It has been reported that K1 cells have their origin in the thyroid papillary carcinoma cell line GLAG-66.

Anwendung

K1 Cell Line human has also been used to study and correlate the expression levels of c-KIT (a receptor tyrosine kinase) and two markers of thyrocyte differentiation (PAX8 and TTF-1).

Tumourigenicity studies, particularly the role of p53

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11,12
D5S818: 10,11
D7S820: 11
THO1: 6,9
TPOX: 8
vWA: 17,18

Nährmedium

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x1,000,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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