GS-109-V-20

90110505, human skin, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: GS-109-V-20, 90110505
• Biologische Quelle: human skin
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian skin fibroblast Gardner′s syndrome

Beschreibung der Zelllinie

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

Anwendung

Tumourigenicity studies

Nährmedium

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

Sonstige Hinweise

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