NCTC 4093

90102530, mouse embryo, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: NCTC 4093, 90102530
• Biologische Quelle: mouse embryo
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 71-73; hypotetraploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C57B1/KaLw embryo

Beschreibung der Zelllinie

Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.

Anwendung

Transformation and virus studies: Polyoma virus

Nährmedium

NCTC 135 100% + 2mM Glutamine.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical methods to dislodge the cells (e.g. scraping); 5% CO₂; 37°C.

Sonstige Hinweise

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