Clone 81

90031403, feline kidney, Fibroblast

• UNSPSC-Code: 41106514
• Biologische Quelle: feline kidney
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer

Eigenschaften

• Produktbezeichnung: Clone 81, 90031403
• Biologische Quelle: feline kidney
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Cat kidney fibroblast, MSV transformed

Beschreibung der Zelllinie

Susceptible to Feline leukaemia virus. There is the possibility of low level secretion of Feline endogenous retrovirus (RD 114). The cells should be handled under laboratory containment level 2.

Anwendung

Virus and cloning studies: FeLV, Feline endogenous retrovirus

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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