CCRF S-180 II

90011901, mouse unknown/unspecified, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: CCRF S-180 II, 90011901
• Biologische Quelle: mouse unknown/unspecified
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 40
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse CFW sarcoma 180

Beschreibung der Zelllinie

Established from an S-180 sarcoma maintained in adult CFW mice. The cells have been used extensively in chemotherapy screening tests.

Anwendung

Chemotherapy screening tests

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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