R2C

89031606, rat testis, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: R2C, 89031606
• Biologische Quelle: rat testis
• Verpackung: tube of 5 μg 89031606-DNA-5UG; pkg of vial of cells 89031606-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat testis Leydig cell tumour

Beschreibung der Zelllinie

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Nährmedium

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO₂; 37°C.

Sonstige Hinweise

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