CRI-G5

88031603, rat pancreas, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CRI-G5, 88031603
• Biologische Quelle: rat pancreas
• Verpackung: tube of 5 μg 88031603-DNA-5UG; pkg of vial of cells 88031603-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Insulin and glucagon
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat NEDH islet tumour

Beschreibung der Zelllinie

Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (Sigma Catalogue number. 88031604).

Anwendung

Hormone studies

Nährmedium

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. The cells tend to grow in clumps and ribbons.

Sonstige Hinweise

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