AC2

86061902, mouse blood, Lymphoblast

• UNSPSC-Code: 41106514
• Biologische Quelle: mouse blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Interleukin 3 (IL-3)

Eigenschaften

• Produktbezeichnung: AC2, 86061902
• Biologische Quelle: mouse blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Interleukin 3 (IL-3)
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse lymphoblast

Beschreibung der Zelllinie

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

Anwendung

Growth factor studies, IL-2 assays

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO₂; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

Sonstige Hinweise

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