Do-11-10

85082301, mouse unknown/unspecified, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: Do-11-10, from mouse unknown/unspecified, 85082301
• Biologische Quelle: mouse unknown/unspecified
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

Beschreibung der Zelllinie

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

Nährmedium

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

Sonstige Hinweise

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