OX-22

84112013, Reactivity: Anti-CD45, rat

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myelom: NS1
• Reaktivität: Anti-CD45, rat
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse NS1 x Mouse BALB/c spleen

Beschreibung der Zelllinie

The antibody reacts mainly with high molecular weight (190-220kDa) form of leukocyte common antigen. Labels all B lymphocytes and 50% of bone marrow cells and 2% of thymocytes. The antibody is directed against CD8+ve T, NK and 75% of CD4+ve cells. Same distribution as OX-32: antibodies do not compete.

Immunogen

PHA activated rat lymph node cells

Nährmedium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.