RCP

00031626

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Endothelial
• Produkte: Not specified
• Rezeptoren: Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Choroid Plexus

Beschreibung der Zelllinie

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

Anwendung

Toxicology, cellular signalling and pharmacology studies

Nährmedium

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.1% Epidermal Growth Factor(EGF) (E9644), 1% Insulin–Transferrin–Selenium(ITS) (I3146)

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.