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CB0500
CelLytic™ B Plus Kit
For bacterial lysis
Synonym(e):
Lysis and protein extraction kit
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About This Item
Empfohlene Produkte
Qualitätsniveau
Verwendung
kit sufficient for 50 g fresh or frozen cell paste lysis.
Methode(n)
protein extraction: suitable
Versandbedingung
wet ice
Lagertemp.
−20°C
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Allgemeine Beschreibung
The CelLytic™ B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.
Anwendung
This kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chromatography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.
Leistungsmerkmale und Vorteile
- Lyse Gram positive and Gram negative bacteria
- More efficient than sonication
- Compatible with affinity purification
- Isolate inclusion bodies for subsequent solubilization
- Non-denaturing cell lysis preserves protein function
Sonstige Hinweise
The CelLytic™ B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.
Rechtliche Hinweise
Benzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- B7435CelLytic™ B Cell Lysis Reagent, For bacterial cell lysis, standard strengthSDB
- E1014Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solutionSDB
- P8849Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solutionSDB
- L3790Lysozyme from chicken egg white, 10 mg/mLSDB
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Lagerklassenschlüssel
10 - Combustible liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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