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Key Documents

C-28019

Sigma-Aldrich

Mesenchymal Stem Cell Growth Medium DXF

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.71

Form

liquid

Verpackung

pkg of 500 ml

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

wet ice

Allgemeine Beschreibung

MSC Growth Medium XF 500 ml, ready-to-use, with C-39819 attached to flask. Serum-free and xeno-free cell culture medium for proliferation of mesenchymal stem cells. Fibronectin coating required.

Anwendung

The Mesenchymal Stem Cell Growth Medium DXF is a defined and xeno-free culture medium for routine culture of multipotent human Mesenchymal Stem Cells. Rigorous quality control tests ensure reliable and consistent product quality.Recommended for:Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)Note: MSC Growth Medium DXF does not contain attachment- and spreading factors. Therefore, culture vessels must be precoated with 10 μg/ml human Fibronectin according to the instruction manual.

Qualität

All lots of PromoCell Mesenchymal Stem Cell Media are subjected to comprehensive quality control tests using primary human mesenchymal stem cells. Each lot of MSC Growth Medium is checked for maintenance of multipotency, growth promoting activity, adherence rate, and typical morphology of the tested mesenchymal stem cells. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Lagerung und Haltbarkeit

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the complete MSC Growth Medium DXF is stable for 3 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subkultur-Routine

Click here for more information.

Lagerklassenschlüssel

10 - Combustible liquids

Analysenzertifikate (COA)

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Artikel

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Protokolle

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Mesenchymal Stem Cells and Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue. Protocols for handling, cell passaging, media, and product use.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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