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Merck

C-12620

Sigma-Aldrich

Human Nasal Epithelial Cells (HNEpC)

500,000 cryopreserved cells

Synonym(e):

HNEpC cells

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About This Item

UNSPSC-Code:
41106514
NACRES:
NA.81

Biologische Quelle

human nose (nasal mucosa)

Verpackung

pkg of 500,000 cells

Morphologie

(epithelial)

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

dry ice

Lagertemp.

−196°C

Allgemeine Beschreibung

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Ursprung der Zelllinie

Nose

Anwendung

Primary Human Nasal Epithelial Cells (HNEpC) are isolated from normal human nasal mucosa and are provided in a cryopreserved format. HNEpC stain positive for cytokeratin. The epithelium in the nasal cavity cleans, humidifies, and warms inhaled air. In addition, it produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. HNEpC are useful for in vitro studies of these processes.

Qualität

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subkultur-Routine

Click here for more information.

Sonstige Hinweise

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

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Recommended Primary Cell Culture Media:Link

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Silver (Ag) is known to possess antimicrobial properties which is commonly attributed to soluble Ag ions. Here, we showed that Ag nanoparticles (NPs) potently inhibited SARS-CoV-2 infection using two different pseudovirus neutralization assays. We also evaluated a set of Ag

Artikel

Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.

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