B8781

Restriction Enzyme Buffer SB

10 ×

• Synonym(e): Restriction Endonuclease Buffer
• UNSPSC-Code: 12352204
• NACRES: NA.53

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Beschreibung: 10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl₂, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.
• Form: buffered aqueous solution
• Konzentration: 10 ×
• Lagertemp.: −20°C
• SMILES String: CC(C)CC(CNC(Cc1ccccc1)C(N)=O)NC(=O)C(Cc2cnc[nH]2)NC(=O)CNC(=O)C(NC(=O)C(C)NC(=O)C(Cc3c[nH]c4ccccc34)NC(=O)C(CCC(N)=O)NC(=O)C(N)Cc5c[nH]c6ccccc56)C(C)C
• InChI: 1S/C58H77N15O9/c1-32(2)21-39(29-65-46(52(61)76)22-35-13-7-6-8-14-35)69-57(81)48(25-38-28-62-31-67-38)70-50(75)30-66-58(82)51(33(3)4)73-53(77)34(5)68-56(80)47(24-37-27-64-44-18-12-10-16-41(37)44)72-55(79)45(19-20-49(60)74)71-54(78)42(59)23-36-26-63-43-17-11-9-15-40(36)43/h6-18,26-28,31-34,39,42,45-48,51,63-65H,19-25,29-30,59H2,1-5H3,(H2,60,74)(H2,61,76)(H,62,67)(H,66,82)(H,68,80)(H,69,81)(H,70,75)(H,71,78)(H,72,79)(H,73,77)
• InChIKey: YNZJHLFHFNCZIE-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Anwendung

Restriction Enzyme Buffer SB has been used in restriction digestion of PCR amplified product.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable