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B5161

Sigma-Aldrich

Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt

≥90% (TLC), powder

Synonym(e):

Sulfosuccinimidyl biotin

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About This Item

Empirische Formel (Hill-System):
C14H18N3NaO8S2
CAS-Nummer:
119616-38-5
Molekulargewicht:
443.43
MDL-Nummer:
MFCD00078535
UNSPSC-Code:
12352203
PubChem Substanz-ID:
24891813
NACRES:
NA.46

Assay

≥90% (TLC)

Form

powder

Löslichkeit

H2O: 10 mg/mL
DMF: 50 mg/mL

Lagertemp.

−20°C

SMILES String

[Na].OS(=O)(=O)C1CC(=O)N(OC(=O)CCCCC2SCC3NC(=O)NC23)C1=O

InChI

1S/C14H19N3O8S2.Na.H/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);;

InChIKey

NKHCQKKDLWGBRK-UHFFFAOYSA-N

Allgemeine Beschreibung

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Anwendung

Water soluble biotinylation reagent. Typically coupled to primary amine in the pH range 6.5-8.5.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Warning

Gefahreneinstufungen

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Persönliche Schutzausrüstung

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Analysenzertifikate (COA)

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Wei-Bor Tsai et al.
Biotechnology and bioengineering, 98(2), 498-507 (2007-03-28)
Cell adhesion to a scaffold is a prerequisite for tissue engineering. Many studies have been focused on enhancing cell adhesion to synthetic materials that are used for scaffold fabrication. Previously, we showed that immobilization of biotin molecules to chondrocyte surfaces
Wei-Bor Tsai et al.
Biomaterials, 26(16), 3141-3151 (2004-12-18)
Cell adhesion to synthetic biomaterials is a prerequisite for anchorage cell culture and tissue engineering. The current study investigated utilization of an avidin-biotin binding system in enhancing chondrocyte adhesion to tissue culture polystyrene (TCPS). Biotinylated chondrocytes adhered to avidin-coated TCPS
P Pantano et al.
Analytical chemistry, 65(5), 623-630 (1993-03-01)
Dehydrogenase enzymes are immobilized onto carbon-fiber microelectrode surfaces via avidin-biotin technology and a covalently linked hydrophilic tether. The avidin-biotin coupling strategy allows the selectivity of the electrochemical measurement to be easily changed without reoptimizing the immobilization conditions. Optimized derivatization conditions
Dominic Kamps et al.
Cell reports, 33(9), 108467-108467 (2020-12-03)
Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system

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