B2787

Biotinylierter Molekulargewichtsmarker

mol wt 6,500-180,000 Da

• Synonym(e): protein markers, protein molecular weight markers, protein standards
• UNSPSC-Code: 12352200
• eCl@ss: 34058011
• NACRES: NA.25

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Verwendung: vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
• Mol-Gew.: 6,500-180,000 Da
• Verpackung: vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
• Lagertemp.: −20°C

Beschreibung

Anwendung

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.

Komponenten

Dieses Produkt enthält:

• α₂-Makroglobulin (Pferdeserum)
• β-Galactosidase (E. coli)
• Phosphorylase b (Kaninchenmuskel)
• Katalase (Rinderleber)
• Alkoholdehydrogenase (Pferdeleber)
• Carboanhydrase (Rindererythrozyten)
• Trypsininhibitor (Sojabohne)
• Lysozym (Hühnerei)
• Aprotinin (Rinderlunge)

Jedes Vial enthält ca. 33% Protein, 33% NaCl und 33% Saccharose.

Sonstige Hinweise

Enthält neun biotinylierte Proteine (gesamt ca. 0.1 mg).

Rekonstituierung

Jedes Vial wird mit 1 mL 1x Probenpuffer (Produkt-Nr. S3401) rekonstituiert und für 2-3 Min. bei 100 °C inkubiert.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable