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B0519

Sigma-Aldrich

Biotin−Agarose

PBS suspension

Synonym(e):

Vitamin H Agarose

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About This Item

MDL-Nummer:
MFCD00163072
UNSPSC-Code:
41106500
NACRES:
NA.56

Form

PBS suspension

Qualitätsniveau

200

Kennzeichnungsgrad

≥30 mg avidin per mL packed resin
2-6 μmol per mL

Matrix

4% beaded agarose

Matrixaktivierung

epoxy

Matrixanbindung

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

Matrix-Spacer

13 atoms

Anwendung(en)

life science and biopharma

Lagertemp.

2-8°C

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Anwendung

Biotin contains a high affinity for binding to avidin or streptavidin. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins.

Physikalische Form

Suspension in PBS, enthält 0.02% Natriumazid

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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K J Airenne et al.
Gene, 144(1), 75-80 (1994-06-24)
A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
A P Sibler et al.
Journal of immunological methods, 224(1-2), 129-140 (1999-06-05)
In vivo biotinylation of antibody fragments with a gene fusion approach is a realistic alternative to conventional in vitro chemical labelling. We have previously reported the construction of a vector system suitable for the bacterial expression of the binding fragment
Emrah Celik et al.
Journal of molecular recognition : JMR, 25(1), 53-56 (2012-01-04)
Sample-probe contact duration (dwell time) and loading force are two important parameters for the atomic force microscopy (AFM) force spectroscopy measurements of ligand-receptor interaction. A prolonged contact time may be required to initiate ligand-receptor binding as a result of slow
P A Smith et al.
Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet, N., et al.
Journal of Materials Chemistry, 14, 2638-2642 (2004)
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Verwandter Inhalt

Protein Pull-Down

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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