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Merck

AP0100

Sigma-Aldrich

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

Synonym(e):

ALP Activity Kit

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.26

Verwendung

sufficient for 100 tests

Qualitätsniveau

Lagertemp.

2-8°C

Allgemeine Beschreibung

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Anwendung

Alkaline Phosphatase Diethanolamine Activity Kit has been used:
  • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
  • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
  • for determining ALP activity in the bone metastasis model of lung cancer
  • for measuring ALP levels in colon tissue homogenates

Eignung

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Prinzip

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powderSDB

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg proteinSDB

Piktogramme

Health hazardCorrosion

Signalwort

Danger

Gefahreneinstufungen

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Zielorgane

Kidney,Liver,Blood

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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