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Merck

A8582

Sigma-Aldrich

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

Synonym(e):

silicone-based antifoam

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About This Item

UNSPSC-Code:
12161901
NACRES:
NA.85

Qualitätsniveau

Form

emulsion (aqueous)

Konzentration

10% (active silicon)

Methode(n)

cell culture | hybridoma: suitable

Löslichkeit

H2O: soluble (effective hot or cold.)

Dichte

1.0 g/mL at 25 °C

Anwendung(en)

microbiology

Lagertemp.

room temp

Eignung

(Mammalian (suspension))
bacteria (fermentation)

Allgemeine Beschreibung

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Anwendung

Antifoam SE-15 has been used:

  • in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
  • to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
  • as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
  • as a component of autoinduction expression culture.
  • as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Leistungsmerkmale und Vorteile

  • Controls foaming in microbial culture flasks or fermentations
  • Non-toxic to the culture medium
  • Can be pumped to fermenter on an as-needed basis

Komponenten

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Angaben zur Herstellung

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

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Piktogramme

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Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Eye Irrit. 2 - STOT RE 2 Inhalation

Zielorgane

Lungs

WGK

WGK 1


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