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Wichtige Dokumente
A7340
Anti-Glutathion-S-Transferase
IgG fraction of antiserum, buffered aqueous solution
Synonym(e):
Anti-GST
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About This Item
UNSPSC-Code:
12352203
NACRES:
NA.46
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Konjugat
peroxidase conjugate
Antikörperform
IgG fraction of antiserum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Mol-Gew.
antigen 27.5 kDa
Methode(n)
direct ELISA: 1:10,000
western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
Der Antikörper ist sowohl für native als auch denaturierte Formen von Glutathion-S-Transferase aus Schistosoma japonicum spezifisch. Der Anti-GST-Antikörper kann in verschiedenen Immunassays zur Identifizierung der Expression von GST-Fusionsproteinen eingesetzt werden.
Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ.
Spezifität
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.
Immunogen
Rekombinantes GST aus Schistosoma japonicum wird in E. coli exprimiert.
Anwendung
Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in
- immunoblotting
- affinity pull-down assay
- western blotting
- enzyme linked immunosorbent assay (ELISA)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem./physiol. Wirkung
Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Angaben zur Herstellung
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Resp. Sens. 1 - Skin Sens. 1
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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