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A7310
Anti-Water Channel Aquaporin 2 antibody produced in rabbit
IgG fraction of antiserum, lyophilized powder
Synonym(e):
Anti-Aquaporin 2, Aquaporin 2 Detection, Rabbit Anti-Aquaporin 2
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About This Item
Konjugat:
unconjugated
application:
IHC
WB CL
WB CL
Klon:
polyclonal
Speziesreaktivität:
rat, human
citations:
30
Methode(n):
immunohistochemistry: suitable
western blot (chemiluminescent): 1:200-1:1000
western blot (chemiluminescent): 1:200-1:1000
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Konjugat
unconjugated
Antikörperform
IgG fraction of antiserum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
lyophilized powder
Speziesreaktivität
rat, human
Methode(n)
immunohistochemistry: suitable
western blot (chemiluminescent): 1:200-1:1000
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... AQP2(359)
mouse ... Aqp2(11827)
rat ... Aqp2(25386)
Verwandte Kategorien
Allgemeine Beschreibung
Aquaporin 2 (AQP2) exists as a tetramer with C terminus showing multiple conformations and binds two cadmium ions. AQP2 gene is mapped to human chromosome 12q13.12. It is associated with intracellular vesicles.
Aquaporin 2 (AQP2) is a water channel protein that regulates water balance in renal connecting tubules and collecting ducts. AQP2 is activated by anti-diuretic hormone and subsequently facilitates water inflow in renal cells. This protein also interacts with integrin to modulate epithelial cell morphogenesis and cell movement . Anti-Water Channel Aquaporin 2 antibody recognizes aquaporin 2 from rat by immunoblotting. The antibody also reacts with mouse and human AQP2.
Immunogen
synthetic peptide corresponding to amino acids 254-271 of rat or mouse aquaporin 2 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde. The epitope is identical in rat and mouse and highly conserved in human.
Anwendung
Anti-Water Channel Aquaporin 2 antibody is also suitable for use in chemiluminescent western blot (1:200-1:1000), and immunoblot.
Anti-Water Channel Aquaporin 2 antibody produced in rabbit has been used in:
- immunostaining
- immunohistochemistry
- immunofluorescence
- immunoblotting
Immunohistochemistry was performed on 10% formalin-fixed parraffin embedded rat kidney sections using rabbit anti-AQP2 at a concentration of 5μg/ml. Use of anti-AQP2 allowed visualization of the medullary thick ascending limb and collecting ducts.
Biochem./physiol. Wirkung
Aquaporin (AQP2) N-terminal region plays a key role in trafficking. The C-terminal phosphorylation is crucial for the water permeability function of the apical membrane. Also, ubiquitination at lysine 270 is needed for AQP2 sorting and degradation in lysosomes or elimination through exosomes. Mutation in AQP2 gene is implicated in congenital nephrogenic diabetes insipidus (NDI), a water balance disorder. The functional dysregulation of AQP2 is also correlated to preeclampsia, congestive heart failure and liver cirrhosis.
Physikalische Form
Lyophilized from a solution containing phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide.
Angaben zur Herstellung
Isolated on immobilized Protein A
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Khalil El Karoui et al.
Nature communications, 7, 10330-10330 (2016-01-21)
In chronic kidney disease (CKD), proteinuria results in severe tubulointerstitial lesions, which ultimately lead to end-stage renal disease. Here we identify 4-phenylbutyric acid (PBA), a chemical chaperone already used in humans, as a novel therapeutic strategy capable to counteract the
George J Schwartz et al.
The Journal of clinical investigation, 125(12), 4365-4374 (2015-11-01)
The nephron cortical collecting duct (CCD) is composed of principal cells, which mediate Na, K, and water transport, and intercalated cells (ICs), which are specialized for acid-base transport. There are two canonical IC forms: acid-secreting α-ICs and HCO3-secreting β-ICs. Chronic
Alexandra Rieger et al.
PloS one, 11(7), e0158977-e0158977 (2016-07-16)
During nephrogenesis, POU domain class 3 transcription factor 3 (POU3F3 aka BRN1) is critically involved in development of distinct nephron segments, including the thick ascending limb of the loop of Henle (TAL). Deficiency of POU3F3 in knock-out mice leads to
Kuniaki Takata et al.
Histochemistry and cell biology, 130(2), 197-209 (2008-06-21)
Aquaporins (AQPs) are membrane proteins serving in the transfer of water and small solutes across cellular membranes. AQPs play a variety of roles in the body such as urine formation, prevention from dehydration in covering epithelia, water handling in the
Jared Q Gerlach et al.
PloS one, 8(9), e74801-e74801 (2013-09-27)
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomolecules from their cells of origin. Although uEV-associated proteins and RNA have been studied in detail, little information exists regarding uEV glycosylation characteristics. Surface glycosylation profiling by
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