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Merck

A6284

Sigma-Aldrich

Human IgG−Agarose

suspension

Synonym(e):

IgG Agarose

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Konjugat

agarose conjugate

Qualitätsniveau

Form

suspension

Kennzeichnungsgrad

≥5 mg per mL

Matrix

Cross-linked 4% beaded agarose

Matrixaktivierung

cyanogen bromide

Matrix-Spacer

1 atom

Lagertemp.

2-8°C

Allgemeine Beschreibung

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Anwendung

IgG-Agarose is an immuno-adsorbent that can be used to affinity purify antibodies, remove species-specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically, cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgG-Agarose (resin volume). However, the resin to antiserum ratio will vary with individual applications.
Tandem affnitiy purification pulldown was performed in yeast cultures using agarose-linked human IgG. Supernatants were mixed with 30μl of agarose beads overnight.

Physikalische Form

Suspension in 0.5 M NaCl containing preservative.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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A G Norden et al.
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A novel interference with measurements of serum free thyroxine (FT4) caused by rheumatoid factor (RhF) is described. We found misleading, sometimes gross, increases of FT4 results in 5 clinically euthyroid elderly female patients with high RhF concentrations. All 5 patients
Yeon Jeong Kim et al.
Frontiers in plant science, 10, 667-667 (2019-06-14)
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