A3688

Anti-Maus-IgG (Gesamtmolekül)−Alkalische Phosphatase in Ziege hergestellte Antikörper

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(e): Goat Anti-Mouse IgG (whole molecule)−AP
• MDL-Nummer: MFCD00162640
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: goat
• Konjugat: alkaline phosphatase conjugate
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Form: buffered aqueous glycerol solution
• Speziesreaktivität: mouse
• Darf nicht reagieren mit: human
• Methode(n): direct ELISA: 1:30,000; dot blot: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody react with normal mouse serum and mouse IgG using IEP. By ODD, the antiserum is reacts with mouse IgG1, IgG2a, IgG2b, IgG3, IgA and IgM. The conjugate shows no reactivity with human serum proteins by ELISA.

Immunogen

purified mouse IgG

Anwendung

Surfactant Protein A wurde in bronchoalveolärer Flüssigkeit unter Verwendung von an alkalischer Phosphatase konjugiertem Anti-Maus-IgG von der Ziege als Sekundärantikörper bei μg/ml in TBS/Tween mit einer Endkonzentration von 0,5 M NaCl nachgewiesen.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody for immunocytochemistry assays and ELISA.

Human and Murine tumor cell lysate were analyzed for various protein expression by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary for 1 hour at 37 degrees.

Sonstige Hinweise

Antibody adsorbed with human serum proteins.

Physikalische Form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

Angaben zur Herstellung

Adsorbed to reduce background with human samples.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable