A1908

Asialofetuin aus Kälberserum fötal

Type II (Sigma designation)

• MDL-Nummer: MFCD00130561
• UNSPSC-Code: 12352202
• NACRES: NA.61

Eigenschaften

• Biologische Quelle: bovine
• Qualitätsniveau: 200
• Typ: Type II (Sigma designation)
• Form: powder
• Methode(n): activity assay: suitable; inhibition assay: suitable
• Verunreinigungen: salt, essentially free; ≤1.0% N-acetylneuraminic acid
• Löslichkeit: H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Asialofetuin from fetal calf serum is suitable for use:

• in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
• in the inhibition of zona pellucida hardening of mouse oocytes
• as an acceptor in sialyltransferase enzyme activity assay

Das Einbringen von Asialofetuin (AF) in Liposome verstärkt die Abgabe an und die Endozytose von Zellen, die den AF-Rezeptor aufweisen, insbesondere Hepatozyten, signifikant. Dieses bietet einen sehr effizienten Weg für die Gentherapie.

Biochem./physiol. Wirkung

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.

Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.

Angaben zur Herstellung

Enzymatic cleavage using neuraminidase

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)