91228

Trichloressigsäure

BioUltra, ≥99.5% (T)

• Synonym(e): TCA
• Lineare Formel: Cl₃CCOOH
• CAS-Nummer: 76-03-9
• Molekulargewicht: 163.39
• Beilstein: 970119
• EG-Nummer: 200-927-2
• MDL-Nummer: MFCD00004177
• UNSPSC-Code: 12352101
• PubChem Substanz-ID: 57653428
• NACRES: NA.25

Eigenschaften

• Dampfdichte: <1 (vs air)
• Dampfdruck: 1 mmHg ( 51 °C)
• Produktlinie: BioUltra
• Assay: ≥99.5% (T)
• Form: crystals; flakes
• Verunreinigungen: insoluble matter, passes filter test
• Glührückstand: ≤0.05%
• Brechungsindex: n20/D 1.62 (lit.)
• pH-Wert: <1.0 (25 °C, 0.5 M in H₂O)
• bp: 196 °C (lit.)
• mp (Schmelzpunkt): 54-58 °C (lit.)
• Löslichkeit: H₂O: 0.5 M at 20 °C, clear, colorless
• Dichte: 1.62 g/mL at 25 °C (lit.)
• Anionenspuren: chloride (Cl^-): ≤30 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• Kationenspuren: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES String: OC(=O)C(Cl)(Cl)Cl
• λ: 0.5 M in H₂O
• UV-Absorption: λ: 260 nm A_max: 1.60; λ: 280 nm A_max: 0.15
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChIKey: YNJBWRMUSHSURL-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Anwendung

Trichloroacetic acid (TCA) has been used:

• to determine its effect on avian embryos during an exposure window encompassing cardiac specification
• as a cell fixative
• to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
• for the precipitation of acidic ribosomal proteins (RPs)

Traditionell zur Fällung von Proteinen verwendet. Zur Bestimmung der Proteinkonzentration durch quantitative Fällung. Zum Entkalken und als Fixationsmittel in der Mikroskopie verwendet.

Sonstige Hinweise

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

Sicherheitshinweise

• Piktogramme: GHS05,GHS09
• Signalwort: Danger
• H-Sätze: H314,H410
• P-Sätze: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Lagerklassenschlüssel: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• Flammpunkt (°F): >235.4 °F - closed cup
• Flammpunkt (°C): > 113 °C - closed cup
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges