81189

Poly(ethylenglykol)

BioUltra, for molecular biology, 1,000

• Synonym(e): PEG
• Lineare Formel: H(OCH₂CH₂)_nOH
• CAS-Nummer: 25322-68-3
• MDL-Nummer: MFCD00081839
• UNSPSC-Code: 12352104
• PubChem Substanz-ID: 24887680
• NACRES: NA.26

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 100; 200
• Produktlinie: BioUltra
• Form: solid
• Mol-Gew.: M_r 950-1050
• Verunreinigungen: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH-Wert: 5.5-7.0 (25 °C, 50 mg/mL in H₂O)
• Löslichkeit: H₂O: 50 mg/mL at 20 °C, clear, colorless
• Anionenspuren: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• Kationenspuren: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤200 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 50 mg/mL in H₂O
• UV-Absorption: λ: 260 nm A_max: ≤0.10; λ: 280 nm A_max: ≤0.05
• SMILES String: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• InChIKey: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Anwendung

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It has been used as a fusion agent to fuse spleen cells with the myeloma cell line SP2/0.

Sonstige Hinweise

PEG-Fällung von DNA-Fragmenten

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)