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72091

Sigma-Aldrich

NBT-BCIP® -Lösung

BioReagent, suitable as substrate for alkaline phosphatase in dot blots

Synonym(e):

BCIP®/NBT, NBT-X-phosphat

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About This Item

UNSPSC-Code:
12171500
PubChem Substanz-ID:
329763805
NACRES:
NA.21

Qualität

suitable as substrate for alkaline phosphatase in dot blots

Produktlinie

BioReagent

Form

liquid

Löslichkeit

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

2-8°C

SMILES String

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

InChIKey

GDPIIVBVMSORRQ-UHFFFAOYSA-L

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Physikalische Form

Lösung aus 18.8 mg/ml Nitro-blau-tetrazoliumchlorid; 9.4 mg/ml 5-Brom-4-chlor-3-indolyl-phosphat Toluidinsalz in 67% DMSO

Sonstige Hinweise

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity

Rechtliche Hinweise

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

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WGK

WGK 2

Flammpunkt (°F)

152.6 °F - closed cup

Flammpunkt (°C)

67 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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