61048
Abberior® STAR 488, NHS-Ester
for STED application
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About This Item
UNSPSC-Code:
12352200
NACRES:
NA.32
Empfohlene Produkte
Form
solid
Konzentration
≥50.0% (degree of coupling)
Löslichkeit
DMF: 1 mg/mL, clear
Fluoreszenz
λex 510 nm; λem 514-534 nm in PBS, pH 7.4
Lagertemp.
−20°C
Allgemeine Beschreibung
Abberior STAR 488 is the latest development for STED microscopy. It is a very bright green fluorescent dye that can be very effectively excited at the prominent 488 nm laser line. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto™ 488 or Alexa Fluor 488.
STED: The dye can most effectively be depleted in STED microscopy at 585-605 nm.
Abberior STAR 488 is the dye of choice for excitation at 488 nm. Even more, the dye is particularly signed and tested for 2-color STED microscopy in combination with our STAR 440SX rusing a 590nm laser. The dye has been very successfully tested in the Leica 2-color TCS STED CW microscope.
Key Features
Absorption Maximum, λmax: 503 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 524nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 585 - 605 nm
Fluorescence Quantum Yield, η: 0.89 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.9 ns (MeOH)
STED: The dye can most effectively be depleted in STED microscopy at 585-605 nm.
Abberior STAR 488 is the dye of choice for excitation at 488 nm. Even more, the dye is particularly signed and tested for 2-color STED microscopy in combination with our STAR 440SX rusing a 590nm laser. The dye has been very successfully tested in the Leica 2-color TCS STED CW microscope.
Key Features
- STED dye of choice at 488 nm excitation
- Ideal depletion behavior in STED microscopy ~590 nm
- 2-color labeling partner with STAR 440SX for 2-color STED microscopy
Absorption Maximum, λmax: 503 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 524nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 585 - 605 nm
Fluorescence Quantum Yield, η: 0.89 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.9 ns (MeOH)
Anwendung
Anti-Rabbit IgG-Abberior® STAR 488 antibody has been used as a secondary antibody:
- for fluorescent immunohistochemistry in prostate cancer cell lines, PPC-1 and TSU-Pr1
- for immunofluorescence in HeLa and monkey kidney COS-7 cells
- for STED (stimulated emission depletion) microscopy in OLN-t40-α-syn cells (oligodendroglial cells)
Eignung
Designed and tested for fluorescent super-resolution microscopy
Sonstige Hinweise
Rechtliche Hinweise
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
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Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
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