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Key Documents

52412

Sigma-Aldrich

R-Phycoerythrin

BioReagent, passes test for gel electrophoresis

Synonym(e):

R-PE, phycobiliprotein

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About This Item

CAS-Nummer:
11016-17-4
MDL-Nummer:
MFCD00081950
UNSPSC-Code:
12352202
NACRES:
NA.32

Produktlinie

BioReagent

Qualitätsniveau

100

Konzentration

≥10.0 mg/mL (UV)

Methode(n)

electrophoresis: suitable

Fluoreszenz

λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2

Eignung

passes test for gel electrophoresis

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Das Produkt ist suspendiert in 150 mM Natriumphosphat, 60% Ammoniumsulfat, 1 mM EDTA, 1 mM Natriumazid, pH 7.0 und muss vor der Konjugation gegen Konjugationspuffer oder PBS dialysiert werden.

Anwendung

R-Phycoerythrin (R-PE) is useful in the laboratory as a fluorescence-based indicator for the presence of cyanobacteria and a variety of immunofluorescence applications. R-PE is a useful fluoroprobe in various electrophoretic procedures. R-Phycoerythrin (R-PE) proteins detect mercury ions in soil and groundwater samples. Phycoerythrin has been widely used in food, cosmetics, immunodiagnostics, and analytical reagents.

Hinweis zur Analyse

A566/A280 >4.6, A620/A566 <0.03, A566/A498 <1.5

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Xuefei Sun et al.
Journal of chromatography. A, 1216(1), 159-164 (2008-12-17)
Electric field gradient focusing (EFGF) is a technique used to simultaneously separate and concentrate biomacromolecules, such as proteins, based on the opposing forces of an electric field gradient and a hydrodynamic flow. Recently, we reported EFGF devices fabricated completely from
Joerg Martini et al.
Lab on a chip, 12(23), 5057-5062 (2012-10-10)
We describe an optical detection technique that delivers high signal-to-noise discrimination to enable a multi-parameter flow cytometer that combines high performance, robustness, compactness and low cost. The enabling technique is termed "spatially modulated detection" and generates a time-dependent signal as
Kun Tang et al.
Biochimica et biophysica acta, 1817(7), 1030-1036 (2012-04-03)
Phycobilisomes, the light-harvesting antennas in cyanobacteria and red algae, consist of an allophycocyanin core that is attached to the membrane via a core-membrane linker, and rods comprised of phycocyanin and often also phycoerythrin or phycoerythrocyanin. Phycobiliproteins show excellent energy transfer
Radek Sachl et al.
Biophysical journal, 101(11), L60-L62 (2012-01-21)
Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter
David Rizzo et al.
Cytometry. Part B, Clinical cytometry, 84(2), 125-132 (2012-12-12)
Flow cytometry is the reference technique for assessing ZAP70 expression, a marker of poor prognosis in CLL. One of the most common methods is to assess ZAP70 levels in CLL cells by calculating the ratio between ZAP70 mean fluorescence intensities
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