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Merck

21878

Sigma-Aldrich

5(6)-Carboxyfluoreszein-N-hydroxysuccinimidester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym(e):

5(6)-FAM OSu, FLUOS

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About This Item

Empirische Formel (Hill-System):
C25H15NO9
CAS-Nummer:
Molekulargewicht:
473.39
MDL-Nummer:
UNSPSC-Code:
12352108
PubChem Substanz-ID:
NACRES:
NA.32

Qualitätsniveau

Produktlinie

BioReagent

Assay

≥80% (HPLC)

Form

powder

Löslichkeit

DMF: soluble
DMSO: soluble

Fluoreszenz

λex 492 nm; λem 517 nm in DMF

Eignung

suitable for fluorescence

Lagertemp.

−20°C

SMILES String

OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10

InChI

1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

InChIKey

YVTPVJDECOUTJW-UHFFFAOYSA-N

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Anwendung

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.
Fluoreszenzlabel für primäre und sekundäre Amin-Gruppen von Proteinen und anderen Biomolekülen.
Wurde verwendet, um fluoreszierende Tubulin-Derivate herzustellen, sowie für die Herstellung eines Phenytoin-Tracers für die Bestimmung dieses Wirkstoffs in Serum und Plasma mittels Fluoreszenz-Polarisations-Immunoassay (FPIA)

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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