T1438

Todd-Hewitt-Bouillon

suitable for microbiology, NutriSelect^® Plus

• UNSPSC-Code: 41171607
• NACRES: NA.85

Eigenschaften

• Sterilität: non-sterile
• Qualitätsniveau: 200
• Form: powder
• Haltbarkeit: limited shelf life, expiry date on the label
• Hersteller/Markenname: NutriSelect^® Plus
• Methode(n): microbiological culture: suitable
• pH-Wert: 7.8±0.2 (25 °C)
• Anwendung(en): clinical testing; food and beverages; microbiology
• Lagertemp.: 2-25°C
• Eignung: nonselective for Streptococcus spp.

Beschreibung

Allgemeine Beschreibung

Todd Hewitt Broth is used for cultivation of haemolytic streptococci for different serological tests. This medium has been recommended as an alternative type in epidemiologic studies of group A streptococci as well as pathogenic microorganisms. Todd Hewitt Broth medium is very nutritive by the presence of peptic digest of animal tissue and beef heart infusion. Dextrose stimulates haemolysin production. The medium is well buffered by sodium phosphate and sodium carbonate to neutralize the acid produced during dextrose fermentation. This restricts destruction of antigenic streptococcal haemolysin. It is also found that sodium phosphate has a stimulating effect on the pneumococcal growth. Todd Hewitt Broth can be employed as an alternative to serum broth or horse flesh digest broth for the cultivation of streptococci prior to serological typing.

Anwendung

Empfohlen für die Kultivierung von Gruppe A beta-hämolytischen Streptokokken, die in serologischen Studien verwendet werden.

Todd Hewitt broth has been used for the culture of various microorganisms, such as Bacillus subtilis and Streptococcus pneumoniae.

Komponenten

Komponente (g/l)
Rinderherz-Infusion 500.00
Tiergewebe, peptisch verdaut 20.00
Dextrose 2.00
Natriumchlorid 2.00
Natriumphosphat 0.40
Natriumcarbonat 2.50

Angaben zur Herstellung

Suspend 37 g of Todd Hewitt Broth in 1000 ml of distilled water. Mix well and dispense as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Fußnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type N95 (US)