E9379

Stains-All

~95% purity, powder

• Synonym(e): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-yliden)-2-methylpropenyl]-naphtho[1,2-d]thiazoliumbromid, 4,5,4′,5′-Dibenzo-3,3′-diethyl-9-methyl-thiacarbocyaninbromid
• Empirische Formel (Hill-System): C₃₀H₂₇BrN₂S₂
• CAS-Nummer: 7423-31-6
• Molekulargewicht: 559.58
• Beilstein: 3865213
• EG-Nummer: 231-047-7
• MDL-Nummer: MFCD00078788
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24894694
• NACRES: NA.47

Eigenschaften

• product name: Stains-All, ~95%
• Assay: ~95%
• Qualitätsniveau: 200
• Form: powder
• Löslichkeit: chloroform: 40 mg (PLUS 4.0 ml)
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-8°C
• SMILES String: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• InChIKey: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Beschreibung

Anwendung

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Biochem./physiol. Wirkung

Stains-all is suitable for differential staining of nucleic acids and proteins as follows; RNA (bluish purple), DNA (blue) and proteins (red). As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Vorsicht

Bei längerer Lichteinwirkung wird der Nukleinsäure-Farbstoff-Komplex verblassen und nicht mehr sichtbar sein.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)