C4916

5-Carboxyfluorescein-Diacetat

~95% purity (HPLC), powder

• Synonym(e): 5-CFDA, 5-Carboxy-di-O-acetylfluorescein
• Empirische Formel (Hill-System): C₂₅H₁₆O₉
• CAS-Nummer: 79955-27-4
• Molekulargewicht: 460.39
• Beilstein: 68265
• MDL-Nummer: MFCD00036872
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24892738
• NACRES: NA.47

Eigenschaften

• product name: 5-Carboxyfluorescein-Diacetat, ~95% (HPLC)
• Assay: ~95% (HPLC)
• Qualitätsniveau: 200
• Form: powder
• Löslichkeit: chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: −20°C
• SMILES String: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1
• InChI: 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
• InChIKey: WPUZGNPQMIWOHE-UHFFFAOYSA-N

Beschreibung

Anwendung

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Biochem./physiol. Wirkung

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges