93321

TRIS Glycin-Puffer -Lösung

BioUltra, 10× concentrate

• Synonym(e): Blotting-Puffer -Lösung
• UNSPSC-Code: 41105319
• NACRES: NA.25

Eigenschaften

• Produktlinie: BioUltra
• Qualitätsniveau: 100
• Form: liquid
• Qualität: 10× concentrate
• Zusammensetzung: glycine, 1.92 M ; TRIS, 0.25 M
• Verunreinigungen: insoluble matter, passes filter test; proteases, none detected
• Glührückstand: ≤0.2%
• pH-Wert: 8.5-8.7
• Anionenspuren: sulfate (SO₄^2-): ≤50 mg/kg
• Kationenspuren: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; NH₄⁺: ≤200 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: neat
• UV-Absorption: λ: 260 nm A_max: 0.1; λ: 280 nm A_max: 0.1

Beschreibung

Anwendung

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Sonstige Hinweise

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves