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Merck

01696

Sigma-Aldrich

Acrylamid

for Northern and Southern blotting, powder blend

Synonym(e):

2-Propenamid, Acrylsäureamid

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About This Item

Lineare Formel:
CH2=CHCONH2
CAS-Nummer:
Molekulargewicht:
71.08
Beilstein:
605349
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
41105319
eCl@ss:
39031205
PubChem Substanz-ID:
NACRES:
NA.25

Qualität

for molecular biology

Qualitätsniveau

Dampfdichte

2.45 (vs air)

Dampfdruck

0.03 mmHg ( 40 °C)

Produktlinie

BioReagent

Assay

≥99.5% (GC)

Form

powder blend

Methode(n)

electrophoresis: suitable

Verunreinigungen

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

bp

125 °C/25 mmHg (lit.)

mp (Schmelzpunkt)

82-86 °C (lit.)

Löslichkeit

H2O: 0.25 g/mL at 20 °C, clear, colorless

Lagertemp.

2-8°C

SMILES String

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChIKey

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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Allgemeine Beschreibung

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Anwendung

Suitable for making polyacrylamide gels according to established recipes.

Vorsicht

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

Piktogramme

Skull and crossbonesHealth hazard

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Zielorgane

Peripheral nervous system

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

280.4 °F - closed cup

Flammpunkt (°C)

138 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Die Dokumentenbibliothek aufrufen

R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Jonas S Laursen et al.
Journal of the American Chemical Society, 135(7), 2835-2844 (2013-01-25)
Non-natural peptide analogs have significant potential for the development of new materials and pharmacologically active ligands. One such architecture, the β-peptoids (N-alkyl-β-alanines), has found use in a variety of biologically active compounds but has been sparsely studied with respect to
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
Janneke G F Hogervorst et al.
Critical reviews in toxicology, 40(6), 485-512 (2010-02-23)
Since 2002, it is known that the probable human carcinogen acrylamide is present in commonly consumed carbohydrate-rich foods, such as French fries and potato chips. In this review, the authors discuss the body of evidence on acrylamide carcinogenicity from both
Janneke G F Hogervorst et al.
Carcinogenesis, 35(5), 1032-1038 (2014-01-09)
Acrylamide, a probable human carcinogen, is present in heat-treated carbohydrate-rich foods. Epidemiological studies have not shown a clear association between acrylamide intake and colorectal cancer (CRC) risk. This may be due to the molecular heterogeneity in colorectal tumors, which was

Protokolle

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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