PS1019

PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem^®

• Synonym(e): Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr ^183/185), Anti-c-Jun N-terminal Kinase 1&2, Phospho-Specific (Thr/Tyr^183/185)
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: mouse, rat, human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pThr183/pTyr185)
• Angaben zum Gen: human ... MAPK8(5599)

Beschreibung

Allgemeine Beschreibung

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 proteins phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Recognizes the ~49 kDa JNK1 and the ~55 kDa JNK2 phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵ in uv-treated HEK293 cells.

This PhosphoDetect Anti-JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of JNK1/2 (pThr¹⁸³/Tyr¹⁸⁵).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human JNK1 and JNK2

Anwendung

Immunoblotting (1:1000)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
UV-treated HEK293 cells or sorbitol-treated PC12 cells

Sonstige Hinweise

Choudhury, B.K., et al. 2002. J. Immunol.169, 5955.
Barr, R.K., et al. 2001. Int. J. Biochem. Cell. Biol.33, 1047.
Dong, C., et al. 2001. J. Clin. Immunol.21, 253.
Janulis, M., et al. 2001. Mol. Cell. Biol.21, 2235.
Pearce, A.K., et al. 2001. Trends Cell Biol.11, 426.
Tong, T., et al. 2001. Exp. Cell Res.269, 64.
Cobb, M.H., et al. 1999. Prog. Biophys. Mol. Biol.71, 479.
Basu, S., et al. 1998. Oncogene17, 3277.

The immunogen sequence is highly conserved among many species, including mouse, rat, chicken, nematode, and Drosophila, but cross-reactivity has not been determined. The immunogen sequence is also conserved in JNK3, but reactivity has not been detected. Antibody should be titrated for optimal results in individual systems.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1