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Key Documents

PC205L

Sigma-Aldrich

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

lyophilized, Calbiochem®

Synonym(e):

Anti-CGRP

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

serum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

rat, mouse

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze

Isotyp

IgG

Versandbedingung

ambient

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Calca(12310)

Allgemeine Beschreibung

Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.

Immunogen

rat α-calcitonin gene related peptide (α-CGRP)

Anwendung


Free Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)

Warnhinweis

Toxicity: Standard Handling (A)

Rekonstituierung

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.

Hinweis zur Analyse

Positive Control
Rat amygdala or spinal cord

Sonstige Hinweise

Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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