NA03
Anti-PCNA (Ab-1) Mouse mAb (PC10)
liquid, clone PC10, Calbiochem®
Synonym(e):
Anti-Proliferating Cell Nuclear Antigen
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
NACRES:
NA.43
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
PC10, monoclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität
rat, yeast, mouse, human, zebrafish
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
do not freeze
Isotyp
IgG2a
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... PCNA(5111)
zebrafish ... Pcna(30678)
Allgemeine Beschreibung
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).
Immunogen
Epitope: Within amino acids 112-121
recombinant PCNA
Anwendung
Immunoblotting (2.5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Hinweis zur Analyse
Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
Sonstige Hinweise
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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