MHSTCMAG-70K

MILLIPLEX^® Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

• Synonym(e): Luminex^® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay
• UNSPSC-Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Eigenschaften

• Qualitätsniveau: 200
• Speziesreaktivität: mouse
• Hersteller/Markenname: Milliplex^®
• assay range: accuracy: 82-102%; sensitivity: 0.26-11.14 pg/mL; standard curve range: 0.10-400 pg/mL; (IL-4); standard curve range: 0.49-2,000 pg/mL; (IFNγ); standard curve range: 0.49-2,000 pg/mL; (IL-17A); standard curve range: 0.49-2,000 pg/mL; (IL-7); standard curve range: 0.49-2,000 pg/mL; (TNFα); standard curve range: 0.73-3,000 pg/mL; (KC/GROα/CXCL1); standard curve range: 0.98-4,000 pg/mL; (IL-2); standard curve range: 0.98-4,000 pg/mL; (IL-5); standard curve range: 0.98-4,000 pg/mL; (IL-6); standard curve range: 1.34-5,500 pg/mL; (IL-10); standard curve range: 2.93-12,000 pg/mL; (IL-12 (p70)); standard curve range: 3.17-13,000 pg/mL; (LIX); standard curve range: 3.42-14.000 pg/mL; (IL-13); standard curve range: 3.91-16,000 pg/mL; (IL-1α); standard curve range: 3.91-16,000 pg/mL; (IL-1β); standard curve range: 3.91-16,000 pg/mL; (MCP-1/CCL2); standard curve range: 6.1-25,000 pg/mL; (GM-CSF); standard curve range: 7.32-30,000 pg/mL; (MIP-2/CXCL2); inter-assay cv: <15%; intra-assay cv: <10%
• Methode(n): multiplexing: suitable
• Nachweisverfahren: fluorometric (Luminex xMAP)
• Versandbedingung: ambient

Beschreibung

Allgemeine Beschreibung

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Panel Type: Cytokines/Chemokines

Spezifität

Cross Reactivty
There is no detectable cross-reactivity within the panel.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Anwendung

• Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α
• Recommended Sample type: serum, plasma, tissue culture or other media
• Recommended Sample dilution: neat or 1:2 diluted serum or plasma
• Assay Run Time: Overnight
• Research Category: Inflammation & Immunology
• Research Subcategory: Immune Response Metabolic Disorders Chronic Inflammatory Disease Cardiovascular Disease

Leistungsmerkmale und Vorteile

Design your multiplex kit by choosing available analytes within this panel.

Lagerung und Haltbarkeit

Recommended storage for kit components is 2 - 8°C.

Sonstige Hinweise

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see protocol for sensitivity of individual analytes.

Rechtliche Hinweise

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS08,GHS05,GHS07,GHS09
• Signalwort: Danger
• H-Sätze: H302 + H312 + H332,H317,H318,H373,H411
• P-Sätze: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Gefahreneinstufungen: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Zielorgane: Respiratory Tract
• Lagerklassenschlüssel: 10 - Combustible liquids