MABT892

Anti-SUN1 Antibody, clone 12.10F

clone 12.10F, from mouse

• Synonym(e): SUN domain-containing protein 1, Protein unc-84 homolog A, Sad1/unc-84 protein-like 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 12.10F, monoclonal
• Speziesreaktivität: mouse, rat
• Darf nicht reagieren mit: human
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_077771
• UniProt-Hinterlegungsnummer: Q9D666
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Sun1(77053)

Beschreibung

Allgemeine Beschreibung

SUN domain-containing protein 1 (UniProt Q9D666; also known as Protein unc-84 homolog A, Sad1/unc-84 protein-like 1) is encoded by the Sun1 (also known as Unc84a) gene (Gene ID 77053) in murine species. SUN1 is a component of the SUN-protein-containing complexes known as LINC (linker of nucleoskeleton and cytoskeleton), which link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis. It also participates in anchoring chromosome movement in the prophase of meiosis and in selective gene expression of coding and non-coding RNAs required for gametogenesis. Additionally, it has a vital role in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing.

Spezifität

Clone 12.10F was raised against a fusion construct composed of murine SUN1 exon 6-, 8-, and 10-encoded regions that are present in murine spliced isoforms 1, 3, and 4 reported by UniProt (Q9D666). The epitope location among these three regions has not been mapped and reactivity toward murine spliced isoform 3 is expected only if the epitope resides within exon 1- or 4-encoded region.

Immunogen

GST-tagged recombinant fusion protein composed of murine SUN1 regions encoded by exons 6, 8, and 10.

Anwendung

Research Category
Zellstruktur

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SUN1 nuclear immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized C2C12 murine myoblasts.

Immunocytochemistry Analysis: A representative lot immunostained nucleus of C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

Western Blotting Analysis: A representative lot detected greatly reduced SUN1 isoforms target bands in lysate from SUN1 siRNA-treated C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

This mouse monoclonal Anti-SUN1 Antibody, clone 12.10F, Cat. No. MABT892 is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN1.

Qualität

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN1 in 10 µg of C2C12 murine myoblast lysate.

Zielbeschreibung

~102 kDa oberved. 102.0/88.20/97.92/94.51 kDa (mouse isoform 1/2/3/4) calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified.

Format: Purified

Purified mous IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable