MABT398

Anti-CD226/DNAM-1 Antibody, clone LeoA1 (Azide Free)

clone LeoA1, from mouse

• Synonym(e): CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD_antigen=CD226
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: LeoA1, monoclonal
• Speziesreaktivität: human
• Methode(n): activity assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_006557
• UniProt-Hinterlegungsnummer: Q15762
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CD226(10666)

Beschreibung

Allgemeine Beschreibung

CD226/DNAM1 is a T cell expressed plasma membrane receptor protein that plays multiple roles in lymphocyte signaling, intercellular adhesion, lymphokine secretion and cytotoxic T-cell and NK cell mediated killing. CD226 activates natural killer (NK) cell-mediated cytotoxicity and is a key player in the immune synapse between killer T cells and targets. Also, because it recognizes CD155 and nectins CD226/DNAM1 is being examined as a potential anti-cancer agent because soluble CD226 directly inhibits tumor growth by binding CD155 expressed on tumor cells and down regulating proliferation. CD226/DNAM-1 is expressed on Th1, but not Th2 or Th0 cells, it is also expressed on NK cells, CD8+ T-cells, but not developing T cells. CD226/DNAM-1 is also expressed on monocytes and platelets.

Spezifität

MABT398 does not work by western blotting under reducing conditions (for this application please see MABT341).

Immunogen

Whole cells corresponding to human CD226/DNAM-1.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Adhäsions-Proteine (CAMs – Zelladhäsionsmoleküle)

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD226/DNAM-1 in CD226 transfected CHO cells and non-transfected CHO cells.
Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected CD226/DNAM-1 in 1X10E6 CD226 transfected CHO cells.
Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet aggregation (Scott, J.L., et al. (1989). Journal of Biol. Chem. 264(23):13475-13482).
Competative Binding Assay Analysis: A representative lot detected CD226/DNAM-1 in the binding of this antibody to resting and pharmacologically activated PHA-induced blasts and Jurkat cells (Sherrington, P.D., et al. (1997). Journal of Biol. Chem. 272:21735-21744).
Inhibition Assay Analysis: A representative lot detected CD226/DNAM-1 which inhibited the induction of both CTL andAK cells from their precursors (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078).
Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in MLA-I44 cells (Burns, G.F., et al. (1985). J. Exp. Med. 161:1063-1078).
Immunoprecipitation Analysis: A representative lot detected CD226/DNAM-1 in Jurkat PTA-1 transfectants stimulated with TPA (Ralston, K.J., et al. (2004). Journal of Biol. Chem. 279:33816-33828).

This Anti-CD226/DNAM-1 Antibody, clone LeoA1 (Azide Free) is validated for use in Western Blotting and Immunocytochemistry and Flow Cytometry and Immunoprecipitation and Inhibition and Activity Assay for the detection of CD226/DNAM-1.

Qualität

Evaluated by Western Blotting in CD226 transfected CHO cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 transfected CHO cell lysate.

Zielbeschreibung

~39 kDa observed

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable