MABT371

Anti-EB1, EB2 and EB3 Antibody, clone KT65

clone KT65, from rat

• Synonym(e): Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, EB2, End binding protein 2, EB3, End binding protein 3, Microtubule-associated protein RP/EB family member 3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: KT65, monoclonal
• Speziesreaktivität: mouse, human
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_031922
• UniProt-Hinterlegungsnummer: Q61166; Q6PER3; Q8R001
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EBF3(253738) , MAPRE2(10982) , MAPRE3(22924)

Beschreibung

Allgemeine Beschreibung

EB (End-binding) proteins are evolutionarily conserved and are important for extending tips of microtubules as well as regulating microtubule dynamics and communication with other intracellular substances. EB1 (End binding protein 1) encoded by the gene EB1/MAPRE1, EB2 (End binding protein 2) encoded by EB2/MAPRE2, and EB3 (End binding protein 3 also known as Microtubule-associated protein RP/EB family member 3) encoded by EB3/RP3/EBF3-S2/EBF3/MAPRE3 bind directly with end-tracking proteins (+TIPs). +TIPs influence microtubule growth, rescue, dynamicity, and suppression. EB1, EB2, and EB3 play major roles in cellular migration and adhesion, and are thought to associate with adenomatous polyposis coli tumor suppressor.

Spezifität

This antibody is expected to detect EB1, EB2 and EB3.

Immunogen

GST-tagged recombinant protein corresponding to mouse EB1, EB2, and EB3.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelettale Signalübertragung

Immunocytochemistry Analysis: A representative lot detected EB1, EB2, and EB3 in NIH/3T3 cells (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).
Immunoprecipitation Analysis: A representative lot immunoprecipitated EB1, EB2, and EB3 in HeLa cell extracts (Van der Vaart, B., et al. (2011). J Cell Biol. 193(6):1083-1099).

This Anti-EB1, EB2 & EB3 Antibody, clone KT65 is validated for use in western blotting, ICC & IP for the detection of EB1.

Qualität

Evaluated by Western Blotting in MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected EB1, EB2, and EB3 in 10 µg of MAPRE1 (EB1), MAPRE2 (EB2), and MAPRE3 (EB3) cell lysate.

Zielbeschreibung

31, 37, 32 kDa calculated. The calculated MW of EB1 (31 kDa), EB2 (37 kDa), and EB3 (32 kDa). The recombinants used to test the reactivity of this antibody to EB1, EB2, and EB3 have the following MW′s. EB1 (56 kDa), EB2 (62 kDa), and EB3 (58 kDa).

Physikalische Form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing PBS.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable